Induction of Calcium Carbonate by Bacillus cereus

	Induction of Calcium Carbonate by Bacillus cereus
	Jinxin Han; Bin Lian; Hongwen Ling
	2013
发表期刊	Geomicrobiology Journal
卷号	30 期号:8 页码:682-689
摘要	The purpose of this research was to study how the bacteria Bacillus cereus (DCB1) utilizes calcium ions in a culture medium with carbon dioxide (CO2) to yield calcium carbonate (CaCO3). The bacteria strain DCB1 was a dominant strain isolated from dolomitic surfaces in areas of Karst topographies. The experimental method was as follows: a modified beef extract-peptone medium (beef extract 3.0g, peptone 10g, NaCl 5.0g, CaCl2 2.0g, glass powder 2.0g, distilled water 1 L, and a pH between 6.5 and 7.5) was inoculated with B. cereus to attempt to induce the synthesis of CaCO3. The sample was then processed by centrifugation every 24h during the 7-day cultivation period. The pH, carbonic anhydrase (CA) activity, and the concentrations of both HCO- (3) and Ca2+ in the supernatant fluid were measured. Subsequently, precipitation in the culture medium was analyzed to confirm, or otherwise, the presence and if present, the formation, of CaCO3. Methods used included X-ray diffraction (XRD), Scanning Electron Microscopy (SEM), Transmission Electron Microscopy (TEM), and Energy Dispersive Spectroscopy (EDS). Meanwhile, the carbon source in the carbonate was classified by its isotope composition. Results showed that B. cereus can improve its pH value in this culture medium; concentrations of HCO- (3) and Ca2+ showed a significant decline over the duration of the cultivation period. CA activity reached its maximum during the second day; XRD, SEM, TEM, and isotope analysis all revealed the presence of CaCO3 as a precipitate. Additionally, these results did not occur in an aseptic control group: no detectable level of CaCO3 was produced therein. In conclusion: B. cereus can metabolize active materials, such as secretase, by its own growth and metabolism, and can either utilize atmospheric CO2, or respire, to induce CaCO3 production. Experimental evidence is offered for a concomitant CO2 reduction and CaCO3 induction by microorganisms.
关键词	Bacillus Cereus Biomineralization Co2 Reduction Calcium Carbonate
学科领域	环境地球化学
收录类别	SCI
语种	英语
文献类型	期刊论文
条目标识符	http://ir.gyig.ac.cn/handle/352002/5065
专题	环境地球化学国家重点实验室_环境地球化学国家重点实验室_期刊论文
推荐引用方式 GB/T 7714	Jinxin Han,Bin Lian,Hongwen Ling. Induction of Calcium Carbonate by Bacillus cereus[J]. Geomicrobiology Journal,2013,30(8):682-689.
APA	Jinxin Han,Bin Lian,&Hongwen Ling.(2013).Induction of Calcium Carbonate by Bacillus cereus.Geomicrobiology Journal,30(8),682-689.
MLA	Jinxin Han,et al."Induction of Calcium Carbonate by Bacillus cereus".Geomicrobiology Journal 30.8(2013):682-689.

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