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Optimization of nutritional constituents for carbonic anhydrase production by Bacillus mucilaginosus K02
GYIG-IR管理员
2011
Source PublicationAfrican Journal of Biotechnology》 , ,
Volume10Issue:42Pages:8403-8413
AbstractThe objective of this study was to optimize the submerged cultured conditions of carbonic anhydrase (CA) production from the strain of Bacillus mucilaginosus K02 by mono-factor tests with blank control and orthogonal design methodology. When the bacteria was cultured under the condition of monofactor tests with blank control and orthogonal designs, and conventional mono-factor test and orthogonal designs, respectively, the CA productions increased from 5.32 µU/L under the basic medium to 34.53 and 26.12 µU/L. The kinetic analysis of 5 L-fermentor showed that the specific production rate of CA in the optimized medium was almost equaled to the one obtained in the basic and optimized medium with conventional methods. The result demonstrated that CA synthesis was positively correlated with cell growth, and the mono-factor tests with blank control applied in the optimizing composition of the media could achieve better results in contrast to the conventional method.
KeywordBacillus Mucilaginosus Carbonic Anhydrase Specific Production Rate Kinetic Analysis Orthogonal
Subject Area环境地球化学
Language英语
Document Type期刊论文
Identifierhttp://ir.gyig.ac.cn/handle/352002/6970
Collection环境地球化学国家重点实验室_环境地球化学国家重点实验室_期刊论文
Recommended Citation
GB/T 7714
GYIG-IR管理员. Optimization of nutritional constituents for carbonic anhydrase production by Bacillus mucilaginosus K02[J]. African Journal of Biotechnology》 , ,,2011,10(42):8403-8413.
APA GYIG-IR管理员.(2011).Optimization of nutritional constituents for carbonic anhydrase production by Bacillus mucilaginosus K02.African Journal of Biotechnology》 , ,,10(42),8403-8413.
MLA GYIG-IR管理员."Optimization of nutritional constituents for carbonic anhydrase production by Bacillus mucilaginosus K02".African Journal of Biotechnology》 , , 10.42(2011):8403-8413.
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