A portable and quantitative biosensor for cadmium detection using glucometer as the point-of-use device | |
Lingwen Zeng; Junyu Gong; Peisen Rong; Chengshuai Liu; Junhua Chen | |
2019 | |
发表期刊 | Talanta |
卷号 | 198页码:412-416 |
摘要 | As an ubiquitous heavy metal pollutant, cadmium ion (Cd2+) is detrimental to food and human health even at low concentrations. Conventional methods require costly instruments and cannot meet the requirements of on-site analysis. Here we report the use of a personal glucose meter (PGM) as the point-of-use (POU) device for portable and quantitative detection of Cd2+. The specific recognition between the aptamer and Cd2+ trigger the recycling signal amplification process by exonuclease III (Exo III). After successive hybridization and cleavage reactions, numerous single-stranded DNA were liberated on the surface of the magnetic bead. An invertase-conjugated DNA that is complementary to the single-stranded DNA is introduced into the sensing system. After magnetic separation, the invertase conjugates hydrolyze sucrose into glucose, thus establishing direct conversion of Cd2+ concentration to glucose amount, which can be directly quantified by a PGM. Thanks to the synergistic signal amplification of Exo III and invertase, the POU device greatly improves the sensitivity for Cd2+ analysis, with a detection limit of 5 p.M. With the advantages of portability, cost-effectiveness, wide availability, and ease of use, the PGM-based detector has the potential to be used by the public as a routine tool for reliable and quantitative detection of Cd2+. |
关键词 | Cadmium Ion biosensor personal Glucose Meter point-of-use signal Amplification |
收录类别 | SCI |
语种 | 英语 |
文献类型 | 期刊论文 |
条目标识符 | http://ir.gyig.ac.cn/handle/42920512-1/10213 |
专题 | 环境地球化学国家重点实验室 |
作者单位 | 1.School of Food Science and Engineering, Foshan University, Foshan 528000, China 2.nstitute of Environment and Food Safety, Wuhan Academy of Agricultural Science, Wuhan 430065, China 3.State Key Laboratory of Environmental Geochemistry, Institute of Geochemistry, Chinese Academy of Sciences, Guiyang 550081, China 4.Guangdong Key Laboratory of Integrated Agro-environmental Pollution Control and Management, Guangdong Institute of Eco-environmental Science & Technology, Guangzhou 510650, China |
推荐引用方式 GB/T 7714 | Lingwen Zeng;Junyu Gong;Peisen Rong;Chengshuai Liu;Junhua Chen. A portable and quantitative biosensor for cadmium detection using glucometer as the point-of-use device[J]. Talanta,2019,198:412-416. |
APA | Lingwen Zeng;Junyu Gong;Peisen Rong;Chengshuai Liu;Junhua Chen.(2019).A portable and quantitative biosensor for cadmium detection using glucometer as the point-of-use device.Talanta,198,412-416. |
MLA | Lingwen Zeng;Junyu Gong;Peisen Rong;Chengshuai Liu;Junhua Chen."A portable and quantitative biosensor for cadmium detection using glucometer as the point-of-use device".Talanta 198(2019):412-416. |
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文件名称/大小 | 文献类型 | 版本类型 | 开放类型 | 使用许可 | ||
A portable and quant(2623KB) | 期刊论文 | 作者接受稿 | 开放获取 | CC BY-NC-SA | 浏览 请求全文 |
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