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A portable and quantitative biosensor for cadmium detection using glucometer as the point-of-use device
Lingwen Zeng;  Junyu Gong;  Peisen Rong;  Chengshuai Liu;  Junhua Chen
2019
发表期刊Talanta
卷号198页码:412-416
摘要

As an ubiquitous heavy metal pollutantcadmium ion (Cd2+) is detrimental to food and human health even at low concentrations. Conventional methods require costly instruments and cannot meet the requirements of on-site analysis. Here we report the use of a personal glucose meter (PGM) as the point-of-use (POU) device for portable and quantitative detection of Cd2+. The specific recognition between the aptamer and Cd2+ trigger the recycling signal amplification process by exonuclease III (Exo III). After successive hybridization and cleavage reactions, numerous single-stranded DNA were liberated on the surface of the magnetic bead. An invertase-conjugated DNA that is complementary to the single-stranded DNA is introduced into the sensing system. After magnetic separation, the invertase conjugates hydrolyze sucrose into glucose, thus establishing direct conversion of Cd2+ concentration to glucose amount, which can be directly quantified by a PGM. Thanks to the synergistic signal amplification of Exo III and invertase, the POU device greatly improves the sensitivity for Cd2+ analysis, with a detection limit of 5 p.M. With the advantages of portability, cost-effectiveness, wide availability, and ease of use, the PGM-based detector has the potential to be used by the public as a routine tool for reliable and quantitative detection of Cd2+.

关键词Cadmium Ion biosensor personal Glucose Meter point-of-use signal Amplification
收录类别SCI
语种英语
文献类型期刊论文
条目标识符http://ir.gyig.ac.cn/handle/42920512-1/10213
专题环境地球化学国家重点实验室
作者单位1.School of Food Science and Engineering, Foshan University, Foshan 528000, China
2.nstitute of Environment and Food Safety, Wuhan Academy of Agricultural Science, Wuhan 430065, China
3.State Key Laboratory of Environmental Geochemistry, Institute of Geochemistry, Chinese Academy of Sciences, Guiyang 550081, China
4.Guangdong Key Laboratory of Integrated Agro-environmental Pollution Control and Management, Guangdong Institute of Eco-environmental Science & Technology, Guangzhou 510650, China
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GB/T 7714
Lingwen Zeng;Junyu Gong;Peisen Rong;Chengshuai Liu;Junhua Chen. A portable and quantitative biosensor for cadmium detection using glucometer as the point-of-use device[J]. Talanta,2019,198:412-416.
APA Lingwen Zeng;Junyu Gong;Peisen Rong;Chengshuai Liu;Junhua Chen.(2019).A portable and quantitative biosensor for cadmium detection using glucometer as the point-of-use device.Talanta,198,412-416.
MLA Lingwen Zeng;Junyu Gong;Peisen Rong;Chengshuai Liu;Junhua Chen."A portable and quantitative biosensor for cadmium detection using glucometer as the point-of-use device".Talanta 198(2019):412-416.
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