GYIG OpenIR  > 环境地球化学国家重点实验室
The delta~(15)N response and nitrate assimilation of Orychophragmus violaceus and Brassica napus plantlets in vitro during the multiplication stage cultured under different nitrate concentrations
Kaiyan Zhang;Yanyou Wu
2017
发表期刊Acta Geochimica
卷号36期号:2页码:190-197
摘要

Natural nitrogen isotope composition (delta~(15)N) is an indicator of nitrogen sources and is useful in the investigation of nitrogen cycling in organisms and ecosystems. delta~(15)N is also used to study assimilation of inorganic nitrogen. However, the foliar delta~(15)N of intact plants, which is a consequence of nitrate assimilation occurring in the roots and shoots, is not suited for studying nitrate assimilation in cases where nitrate is the sole nitrogen source. In this study, Orychophragmus violaceus (Ov) and Brassica napus (Bn) plantlets, in which nitrate assimilation occurred in the leaves, were used to study the relationship between foliar delta~(15)N and nitrate assimilation. The plantlets were grown in vitro in culture media with different nitrate concentrations, and no root formation occurred for the plantlets during the multiplication stage. Nitrogen isotope fractionation occurred in both the Ov and the Bn plantlets under all treatments. Furthermore, the foliar nitrogen content of both the Ov and Bn plantlets increased with increasing nitrate concentration. Foliar nitrogen isotope fractionation was negatively correlated with foliar nitrogen content for both the Ov and Bn plantlets. Our results suggest that the foliar nitrogen isotope fractionation value could be employed to evaluate nitrate assimilation ability and leaf nitrate reductase activity. Moreover, high external nitrate concentrations could contribute to improved foliar nitrogen content and enhanced nitrate assimilation ability.

关键词Delta~(15)n Nitrate Assimilation Nitrogen Isotope Fractionation Nitrogen Content Nitrate Reductase Activity
收录类别EI
语种英语
文献类型期刊论文
条目标识符http://ir.gyig.ac.cn/handle/42920512-1/7759
专题环境地球化学国家重点实验室
作者单位1.Institute of Geochemistry, Chinese Academy of Sciences, State Key Laboratory of Environmental Geochemistry, Guiyang, Guizhou 550081, China
2.University of Chinese Academy of Sciences, Beijing 100049, China
第一作者单位中国科学院地球化学研究所
通讯作者单位中国科学院地球化学研究所
推荐引用方式
GB/T 7714
Kaiyan Zhang;Yanyou Wu. The delta~(15)N response and nitrate assimilation of Orychophragmus violaceus and Brassica napus plantlets in vitro during the multiplication stage cultured under different nitrate concentrations[J]. Acta Geochimica,2017,36(2):190-197.
APA Kaiyan Zhang;Yanyou Wu.(2017).The delta~(15)N response and nitrate assimilation of Orychophragmus violaceus and Brassica napus plantlets in vitro during the multiplication stage cultured under different nitrate concentrations.Acta Geochimica,36(2),190-197.
MLA Kaiyan Zhang;Yanyou Wu."The delta~(15)N response and nitrate assimilation of Orychophragmus violaceus and Brassica napus plantlets in vitro during the multiplication stage cultured under different nitrate concentrations".Acta Geochimica 36.2(2017):190-197.
条目包含的文件
文件名称/大小 文献类型 版本类型 开放类型 使用许可
The δ15N response an(514KB)期刊论文作者接受稿开放获取CC BY-NC-SA浏览 请求全文
个性服务
推荐该条目
保存到收藏夹
查看访问统计
导出为Endnote文件
谷歌学术
谷歌学术中相似的文章
[Kaiyan Zhang;Yanyou Wu]的文章
百度学术
百度学术中相似的文章
[Kaiyan Zhang;Yanyou Wu]的文章
必应学术
必应学术中相似的文章
[Kaiyan Zhang;Yanyou Wu]的文章
相关权益政策
暂无数据
收藏/分享
文件名: The δ15N response and nitrate assimilation of Orychophragmus violaceus and Brassica napus plantlets in vitro during the multiplication stage cultured under different nitrate concentrations.pdf
格式: Adobe PDF
所有评论 (0)
暂无评论
 

除非特别说明,本系统中所有内容都受版权保护,并保留所有权利。