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The delta~(15)N response and nitrate assimilation of Orychophragmus violaceus and Brassica napus plantlets in vitro during the multiplication stage cultured under different nitrate concentrations
Kaiyan Zhang;Yanyou Wu
2017
Source PublicationActa Geochimica
Volume36Issue:2Pages:190-197
Abstract

Natural nitrogen isotope composition (delta~(15)N) is an indicator of nitrogen sources and is useful in the investigation of nitrogen cycling in organisms and ecosystems. delta~(15)N is also used to study assimilation of inorganic nitrogen. However, the foliar delta~(15)N of intact plants, which is a consequence of nitrate assimilation occurring in the roots and shoots, is not suited for studying nitrate assimilation in cases where nitrate is the sole nitrogen source. In this study, Orychophragmus violaceus (Ov) and Brassica napus (Bn) plantlets, in which nitrate assimilation occurred in the leaves, were used to study the relationship between foliar delta~(15)N and nitrate assimilation. The plantlets were grown in vitro in culture media with different nitrate concentrations, and no root formation occurred for the plantlets during the multiplication stage. Nitrogen isotope fractionation occurred in both the Ov and the Bn plantlets under all treatments. Furthermore, the foliar nitrogen content of both the Ov and Bn plantlets increased with increasing nitrate concentration. Foliar nitrogen isotope fractionation was negatively correlated with foliar nitrogen content for both the Ov and Bn plantlets. Our results suggest that the foliar nitrogen isotope fractionation value could be employed to evaluate nitrate assimilation ability and leaf nitrate reductase activity. Moreover, high external nitrate concentrations could contribute to improved foliar nitrogen content and enhanced nitrate assimilation ability.

KeywordDelta~(15)n Nitrate Assimilation Nitrogen Isotope Fractionation Nitrogen Content Nitrate Reductase Activity
Indexed ByEI
Language英语
Document Type期刊论文
Identifierhttp://ir.gyig.ac.cn/handle/42920512-1/7759
Collection环境地球化学国家重点实验室
Affiliation1.Institute of Geochemistry, Chinese Academy of Sciences, State Key Laboratory of Environmental Geochemistry, Guiyang, Guizhou 550081, China
2.University of Chinese Academy of Sciences, Beijing 100049, China
First Author Affilication中国科学院地球化学研究所
Corresponding Author Affilication中国科学院地球化学研究所
Recommended Citation
GB/T 7714
Kaiyan Zhang;Yanyou Wu. The delta~(15)N response and nitrate assimilation of Orychophragmus violaceus and Brassica napus plantlets in vitro during the multiplication stage cultured under different nitrate concentrations[J]. Acta Geochimica,2017,36(2):190-197.
APA Kaiyan Zhang;Yanyou Wu.(2017).The delta~(15)N response and nitrate assimilation of Orychophragmus violaceus and Brassica napus plantlets in vitro during the multiplication stage cultured under different nitrate concentrations.Acta Geochimica,36(2),190-197.
MLA Kaiyan Zhang;Yanyou Wu."The delta~(15)N response and nitrate assimilation of Orychophragmus violaceus and Brassica napus plantlets in vitro during the multiplication stage cultured under different nitrate concentrations".Acta Geochimica 36.2(2017):190-197.
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